Plasmid_Backbone

Part:BBa_K4294091:Design

Designed by: Moore et al, Paul Freemont lab   Group: iGEM22_Athens   (2022-10-12)


pTU1-A-lacZ destination Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2043
    Illegal XbaI site found at 2058
    Illegal XbaI site found at 2087
    Illegal SpeI site found at 2477
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2043
    Illegal SpeI site found at 2477
    Illegal PstI site found at 1
    Illegal NotI site found at 2049
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2043
    Illegal BamHI site found at 2483
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2043
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2087
    Illegal SpeI site found at 2477
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2043
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2058
    Illegal XbaI site found at 2087
    Illegal SpeI site found at 2477
    Illegal PstI site found at 1
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2495
    Illegal BsaI.rc site found at 2081


Design Notes

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Source

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References